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Esterase&Lipase (PLE&CALB)

Short Description:

About Esterase&Lipase

ES-PLEs: A class of hydrolases that catalyze the formation and breakage of ester bonds. They can take part in many kinds of biochemical reactions, such as esterification, transesterification and hydrolysis. They are widely used in many fields such as food processing, chemical industry, biomedicine, chiral medicine and environmental treatment.

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E-Mail: lchen@syncozymes.com


Product Detail

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About Esterase&Lipase:

There are 26 kinds of PLE enzyme products (Number as ES-PLE-101~ES-PLE-126) developed by SyncoZymes. ES-PLE can be used for the hydrolysis of aliphatic and ester compounds, or regioselective and stereoselective resolution to synthesize chiral acids and their derivatives.

Catalytic reaction type:

Esterase&Lipase (PLE&CALB)
Esterase&Lipase (PLE&CALB)1

Advantages:

★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

Instructions for use:

➢ Normally, the reaction system should include substrate, buffer solution and the ES-PLE. The esterification of some ES-PLEs is carried out in the organic phase.
➢ All kinds of ES-PLEs corresponding to various optimum reaction conditions should be studied individually.

Application Examples:

Example 1(Biosynthesis of Pregabalin Intermediate) (1):

calb1

Example 2 (2):

calb2

Example 3 (3):

calb3

Example 4(4):

calb5

Storage:

Keep 2 years below -20℃.

Attention:

Never contact with extreme conditions such as: high temperature, high/low pH.

References:

1. Xu FX, Chen SY, Xu G, e tal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, F.C., Lee, L. F., Mittal, R. S. D. e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, H. J., Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917


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