Factory best selling Nad Plus Powder - Nitrile reductase (NRED) – SyncoZymes
Factory best selling Nad Plus Powder - Nitrile reductase (NRED) – SyncoZymes Detail:
| Enzymes | Product Code | Specification |
| Specification | ES- NRED -101~ ES- NRED -103 | a set of 3 Nitrile Reductase, 50 mg each 3 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml Substrate
10 mg/ml Enzyme
10% (v/v) Organic cosolvent (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC、HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
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By Victor from Madagascar - 2017.02.14 13:19
In China, we have many partners, this company is the most satisfying to us, reliable quality and good credit, it is worth appreciation.
By Quintina from Monaco - 2018.06.19 10:42
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