Factory For Nadh Vitamin B3 - Ketoreductase (KRED) – SyncoZymes

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Factory For Nadh Vitamin B3 - Ketoreductase (KRED) – SyncoZymes Detail:

KRED from SyncoZymes: There are 191 kinds of KRED enzyme products (Number as ES-KRED-101~ES-KRED-291) developed by SyncoZymes. SZ-KRED could be widely used in regio- and stereoselective reduction of aldehydes, beta-ketone esters, α-ketone esters and ketones.

Catalytic reaction type:

Ketoreductase KRED2

Enzymes	Product Code	Specification
Enzyme Powder	Enzyme Powder	a set of 187 Ketoreductases, 50 mg each 187 items * 50mg / item, or other quantity
Screening Kit (SynKit)	ES-KRED-18100	a set of 181 Ketoreductases, 1 mg each 181 items * 1mg / item

★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion efficiency.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Enzyme screening should be carried out for specific substrates because of the substrate specificity, and get an enzyme that catalyzes the target substrate with best catalytic effect.
➢ Never contact with extreme conditions such as: high temperature, high/low pH and organic solvent with high concentration.
➢ Normally, the reaction system should include substrate, buffer solution, coenzymes( NAD(H) or NADP(H) ), coenzyme regeneration system(e.g. glucose and glucose dehydrogenase).
➢ KRED should be added last into reaction system, after the pH and temperature been adjusted to the reaction condition.
➢ All kinds of KRED have various optimum reaction conditions, so each of them should be further studied individually.

Example 1⁽¹⁾:

Ketoreductase KRED3

Example 2⁽²⁾:

Ketoreductase KRED4

1. Li Z, Liu W D, Chen X, e tal. Tetrahedron, 2013, 69, 3561-3564.
2. Ni Y, Li C X, Ma H M, e tal. Appl Microbiol Biotechnol, 2011, 89: 1111-1118.

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