Hot Selling for Nadh Innoval - Nitrile reductase (NRED) – SyncoZymes
Hot Selling for Nadh Innoval - Nitrile reductase (NRED) – SyncoZymes Detail:
Enzymes | Product Code | Specification |
Specification | ES- NRED -101~ ES- NRED -103 | a set of 3 Nitrile Reductase, 50 mg each 3 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml Substrate
10 mg/ml Enzyme
10% (v/v) Organic cosolvent (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC、HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
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By Monica from Pretoria - 2017.08.21 14:13
The factory can meet continuously developing economic and market needs, so that their products are widely recognized and trusted, and that's why we chose this company.
By Mignon from Borussia Dortmund - 2017.03.08 14:45
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