Hydroaminase (HAM)
Short Description:
About Hydroaminase
There are 2 kinds of HAM enzyme product (Number as ES-HAM-101~ ES-HAM-102) developed by SyncoZymes. HAM can catalyze the ammoniation of enoic acid or its derivatives to produce chiral amino acids. HAM can be used to synthesize chiral amino acids (or chiral amines) from enoic acids (or alkenes). Ammonia donor, such as ammonia water or ammonium salt, is necessary.
Hydroaminase catalytic reaction type
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| Enzymes | Product Code | Specification |
| Enzyme Powder | ES-HAM-101~ ES-HAM-102 | a set of 2 Nuclosidephosphoryalse, 50 mg each 2 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
10% (v/v): Ammonia
Adjust pH to 9.0 before adding enzyme, and enzyme should be added finally. Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC、HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
