Manufacturer of Nadph To Nadp - Nitrile reductase (NRED) – SyncoZymes
Manufacturer of Nadph To Nadp - Nitrile reductase (NRED) – SyncoZymes Detail:
| Enzymes | Product Code | Specification |
| Specification | ES- NRED -101~ ES- NRED -103 | a set of 3 Nitrile Reductase, 50 mg each 3 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml Substrate
10 mg/ml Enzyme
10% (v/v) Organic cosolvent (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC、HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
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By Nancy from Seattle - 2017.04.28 15:45
The company can keep up with the changes in this industry market, product updates fast and the price is cheap, this is our second cooperation, it's good.
By Christine from Colombia - 2018.09.12 17:18
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