Nucloside phosphoryalse (NP)

Short Description:

About nucleoside phosphoryalse

There are 3 kinds of NP enzyme products (Number as ES-NP-101~ ES-NP-103) developed by SyncoZymes. ES-NP-101 is purine nucleoside phosphorylase, ES-NP-102 and ES-NP-103 are pyrimidine nucleoside phosphorylase. Nucleosidephosphorylase could decompose nucleosides into bases and pentose phosphate. Nucleoside phosphorylase can be divided into purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase according to the preference of nucleoside bases. Purine nucleoside phosphorylase can metabolize adenosine to adenine, inosine to hypoxanthine, guanosine to guanine, and produce ribose phosphate at the same time. Pyrimidine nucleoside phosphorylase can metabolize uridine to uracil and produce ribose phosphate.

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Product Detail

Product Tags

Nucloside phosphoryalse reaction type:

Product Information:

Enzymes	Product Code	Specification
Enzyme Powder	ES-NP-101~ ES-NP-103	a set of 3 Nuclosidephosphoryalse, 50 mg each 3 items * 50mg / item, or other quantity

Nucloside phosphoryalse screening reactions procedure:

The following reaction system could be used for reference:
1-10 mg/ml Substrate
10 mg/ml Enzyme
100 mM Phosphate buffer (pH7.0)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 12~24 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC, HPLC or GC analysis.