Renewable Design for Apis Cdmo - Esterase&Lipase (PLE&CALB) – SyncoZymes

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Renewable Design for Apis Cdmo - Esterase&Lipase (PLE&CALB) – SyncoZymes Detail:

There are 26 kinds of PLE enzyme products (Number as ES-PLE-101～ES-PLE-126) developed by SyncoZymes. ES-PLE can be used for the hydrolysis of aliphatic and ester compounds, or regioselective and stereoselective resolution to synthesize chiral acids and their derivatives.

Catalytic reaction type:

Esterase&Lipase (PLE&CALB)
Esterase&Lipase (PLE&CALB)1

★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Normally, the reaction system should include substrate, buffer solution and the ES-PLE. The esterification of some ES-PLEs is carried out in the organic phase.
➢ All kinds of ES-PLEs corresponding to various optimum reaction conditions should be studied individually.

Example 1(Biosynthesis of Pregabalin Intermediate)⁽¹⁾:

calb1

Example 2 ⁽²⁾:

calb2

Example 3⁽³⁾:

calb3

Example 4⁽⁴⁾:

calb5

Keep 2 years below -20℃.

Never contact with extreme conditions such as: high temperature, high/low pH.

1. Xu FX, Chen SY, Xu G, e tal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, F.C., Lee, L. F., Mittal, R. S. D. e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, H. J., Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917

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