Short Lead Time for Nadh Nadp - Esterase&Lipase (PLE&CALB) – SyncoZymes
Short Lead Time for Nadh Nadp - Esterase&Lipase (PLE&CALB) – SyncoZymes Detail:
There are 26 kinds of PLE enzyme products (Number as ES-PLE-101～ES-PLE-126) developed by SyncoZymes. ES-PLE can be used for the hydrolysis of aliphatic and ester compounds, or regioselective and stereoselective resolution to synthesize chiral acids and their derivatives.
Catalytic reaction type:
★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.
➢ Normally, the reaction system should include substrate, buffer solution and the ES-PLE. The esterification of some ES-PLEs is carried out in the organic phase.
➢ All kinds of ES-PLEs corresponding to various optimum reaction conditions should be studied individually.
Example 1(Biosynthesis of Pregabalin Intermediate) (1):
Example 2 (2):
Example 3 (3):
Example 4(4):
Keep 2 years below -20℃.
Never contact with extreme conditions such as: high temperature, high/low pH.
1. Xu FX, Chen SY, Xu G, e tal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, F.C., Lee, L. F., Mittal, R. S. D. e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, H. J., Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917
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