Short Lead Time for Nadh Nadp - Leucine Dehydrogenase (LeuDH) – SyncoZymes
Short Lead Time for Nadh Nadp - Leucine Dehydrogenase (LeuDH) – SyncoZymes Detail:
Enzymes | Product Code | Specification |
Enzyme Powder | ES-LeuDH-101 | a set of 1 Monoamine Oxidase, 50 mg each 1 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC, HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
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By Laura from Anguilla - 2017.09.22 11:32
This enterprise in the industry is strong and competitive, advancing with the times and develop sustainable, we are very pleased to have a opportunity to cooperate!
By Julia from Senegal - 2017.04.18 16:45
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