Special Design for Nmn Supplement Gmp - Hydroaminase (HAM) – SyncoZymes
Special Design for Nmn Supplement Gmp - Hydroaminase (HAM) – SyncoZymes Detail:
| Enzymes | Product Code | Specification |
| Enzyme Powder | ES-HAM-101~ ES-HAM-102 | a set of 2 Nuclosidephosphoryalse, 50 mg each 2 items * 50mg / item, or other quantity |
The following reaction system could be used for reference:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
10% (v/v): Ammonia
Adjust pH to 9.0 before adding enzyme, and enzyme should be added finally. Incubate the reactions at a constant temperature shaker (for example, 30oC, 150rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC、HPLC or GC analysis.
Note (1): Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.
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