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Transaminase (ATA)

Short Description:

About transaminase

ATA from SyncoZymes: There are 66 kinds of ATA enzyme products (Number as ES-ATA-101~ES-ATA-166) developed by SyncoZymes. SZ-ATA is a useful tool to catalyze regio- and stereoselective synthesis of chiral amines, amino acids and their derivatives from a variety of aliphatic and aromatic ketoacids, aldehydes, ketones and ketoses.

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E-Mail: lchen@syncozymes.com


Product Detail

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About transaminase:

Enzymes: Are macromolecular biological catalysts, most enzymes are proteins.

Transaminases: A class of enzymes that catalyze amino transferring between amino acids and keto acids. Transaminases are key biological enzymes in asymmetric synthesis and racemic resolution of chiral amines.
Aminotransferase can be divided into four classes according to sequence and structure: Ⅰ, Ⅱ, Ⅲ and Ⅳ. ω-aminotransferases belong to class II transaminases, commonly be used in the preparation of chiral amines and unnatural amino acids, such as β-amino acids.

ω-aminotransferases: In most cases, ω-transaminase refers to a class of enzymes, that catalytic ammonia transfer reactions without α-amino acid as substrate or product.

Catalytic mechanism:

Transaminase ATA1

Product Information:

Enzymes Product Code Product Code
Enzyme Powder ES-ATA-101~ ES-ATA-165 a set of 65 ω-Transaminases, 50 mg each 65 items * 50mg / item, or other quantity
Screening Kit (SynKit) ES-ATA-6500 a set of 65 ω-Transaminases, 1 mg each 65 items * 1mg / item

Advantages of ATA for biotransformation:

★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion efficiency.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

Instructions for use:

➢ Enzyme screening should be carried out for specific substrates because of the substrate specificity, and get an enzyme that catalyzes the target substrate with best catalytic effect.
➢ Never contact with extreme conditions such as: high temperature, high/low pH and organic solvent with high concentration.
➢ Normally, the reaction system should include substrate, buffer solution, amino donor (such as amino acids and 1-phenyl ethylamine) or receptor (such as keto acids), coenzyme (PLP), cosolvent (such as DMSO).
➢ ATA should be added last into reaction system, after the pH and temperature been adjusted to the reaction condition.
➢ All kinds of ATA have various optimum reaction conditions, so each of them should be further studied individually.

Application Examples:

Example 1(synthesis of Sitagliptin, asymmetric synthesis) (1):

Transaminase ATA2

Example 2 (Mexiletine, Combination of kinetic resolution with asymmetric synthesis)(2):

Transaminase ATA3

References:

1 Savile CK, Janey JM, Mundorff EC, et al. Science, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al. Organic letters, 2009,11(21):4810-4812.


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