SyncoZymes

products

Wholesale Price Niacinamide Adenine Dinucleotide - Amidase (AMD) – SyncoZymes

Short Description:


Product Detail

Product Tags

Related Video

Feedback (2)

We support our purchasers with ideal premium quality products and substantial level company. Becoming the specialist manufacturer in this sector, we've acquired rich practical working experience in producing and managing for Nmn Powder Suppliers , Joe Rogan Nmn Supplement , Nmn Supplement Joe Rogan , We glance forward to giving you with our solutions while in the in the vicinity of future, and you will come across our quotation may be very affordable and the top quality of our merchandise is extremely outstanding!
Wholesale Price Niacinamide Adenine Dinucleotide - Amidase (AMD) – SyncoZymes Detail:

Enzymes: Are macromolecular biological catalysts, most enzymes are proteins
Amidase: Catalyze the hydrolysis of various endogenous and foreign aliphatic and aromatic amides by transferring an acyl group to water with the production of free acids and ammonia. Hydroxamic acids and other organic acids are widely used as drugs because they are constituents of growth factors, antibiotics and tumor inhibitors. The amidases can be divided into R type and S type acylases according to the catalyst stereoselectivity.

In addition to catalyzing the hydrolysis of amides, amidase can also catalyze acyl transfer reactions in the presence of co-substrates such as hydroxylamine.

Amidase with different sources have different substrate specificity, some of them can only hydrolyze aromatic amides, some of them can only hydrolyze aliphatic amides, and some hydrolyze α-or ω-amino amides. Most of the amines have good catalytic activity only for acyclic or simple aromatic amides, but for complex aromatics, heterocyclic amides, especially amides with ortho substituents, are generally low in activity (only a few enzymes exhibit better catalytic effects).

Catalytic mechanism:

amd
Amidase (AMD)2

Enzymes Product Code Product Code
Enzyme Powder ES-AMD-101~ ES-AMD-119 a set of 19 amidases, 50 mg each 19 items * 50mg / item, or other quantity
Screening Kit (SynKit) ES-AMD-1900 a set of 19 amidases, 1 mg each 19 items * 1mg / item

★ High substrate specificity.
★ Strong chiral selectivity.
★ High conversion efficiency.
★ Less by-products.
★ Mild reaction conditions.
★ Environmentally friendly.

➢ Enzyme screening should be carried out for specific substrates because of the substrate specificity, and get an enzyme that catalyzes the target substrate with best catalytic effect.
➢ Never contact with extreme conditions such as: high temperature, high/low pH and organic solvent with high concentration.
➢ Normally, the reaction system should include substrate, buffer solution (The optimum reaction pH of enzyme). Co-substrates such as hydroxylamine should be presence in acyl transfer reaction system.
➢ AMD should be added last into reaction system with optimum reaction pH and temperature.
➢ All kinds of AMD have various optimum reaction conditions, so each of them should be further studied individually.

Example 1 (1):

Hydrolysis activity to different Amide Substrates

Substrate

Specific activity

μmols min-1mg-1

Substrate

Specific activity

μmols min-1mg-1

Acetamide

3.8

ο-OH benzamide

1.4

Propionamide

3.9

p-OH benzamide

1.2

Lactamide

12.8

ο-NH2 benzamide

1.0

Butyramide

11.9

p-NH2 benzamide

0.8

Isobutyramide

26.2

ο-Toluamide

0.3

Pentanamide

22.0

p-Toluamide

8.1

Hexanamide

6.4

Nicotinamide

1.7

Cyclohexanamide

19.5

Isonicotinamide

1.8

Acrylamide

10.2

Picolinamide

2.1

Metacrylamide

3.5

3-Phenylpropionamide

7.6

Prolinamide

3.4

Indol-3-acetamide

1.9

Benzamide

6.8

   

The reaction was carried out in 50mM sodium phosphate buffer solution, pH 7.5, at 70 ℃.

Amides

Hydroxylamine

Hydrazine

Acetamide

8.4

1.4

Propionamide

18.4

3.0

Isobutyramide

25.0

22.7

Benzamide

9.2

6.1

The reaction was carried out in 50mM sodium phosphate buffer solution, pH 7.5, at 70 ℃.
Related reagent concentration: amides, 100 mM(benzamide, 10 mM); hydroxylamine and hydrazine, 400 mM; enzyme 0.9 μM.

Example 2 (2):

Example 2

Example 3 (3):

Example 3

1. D’Abusco A.S., Ammendola S., et al. Extremophiles, 2001, 5:183-192.
2. Guo F M, Wu J P, Yang L R, et al. Process Biochemistry, 2015, 50(8): 1400-1404.
3. Zheng R C, Jin J Q, Wu Z M, et al. Bioorganic Chemistry, 2017, Available online 7.


Product detail pictures:

Wholesale Price Niacinamide Adenine Dinucleotide - Amidase (AMD)  – SyncoZymes detail pictures


Related Product Guide:

We continuously execute our spirit of ''Innovation bringing development, Highly-quality ensuring subsistence, Management advertising and marketing gain, Credit history attracting buyers for Wholesale Price Niacinamide Adenine Dinucleotide - Amidase (AMD) – SyncoZymes , The product will supply to all over the world, such as: San Diego, Detroit, Sao Paulo, We have a good reputation for stable quality products, well received by customers at home and abroad. Our company would be guided by the idea of "Standing in Domestic Markets, Walking into International Markets". We sincerely hope that we could do business with customers both at home and abroad. We expect sincere cooperation and common development!
  • After the signing of the contract, we received satisfactory goods in a short term, this is a commendable manufacturer.
    5 Stars By Mary from United Kingdom - 2017.09.28 18:29
    This supplier stick to the principle of "Quality first, Honesty as base", it is absolutely to be trust.
    5 Stars By Patricia from Finland - 2017.03.07 13:42
    Write your message here and send it to us